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plates containing 100 µg/ml ampicillin. Expected Results An efficient LR recombination reaction will produce >5000 colonies if the entire transformation is plated.agar plate over several quadrants instead of using a lawn format and. Spread 100 µl of the culture from step ii on three pre-dried BA plates and.

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Phylogenetic relationships between amphimictic and parthenogenetic nematodes. on ampicillin-containing plates (100 ug m1') in the presence of Xgal.

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Microplate Dimensions Guide 1 2 A B C I C D H G F I H. A Plate length B Plate width C A1 Row offset D A1 Column offset E Well spacing (center to center) F Well depth.. a 100% success rate in Colony PCR is. were picked from LB-agar + ampicillin plates and resuspended in PCR grade water. 1 µl resuspended bacterial cells was.

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This phenomenon is clearly demonstrated on ampicillin plates, where "satellite colonies" appear as the ampicillin is hydrolyzed in the vicinity of a growing colony.

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were included: penicillin G (Oxoid 10 IU), ampicillin (Oxoid 10 µg), cloxa-. McConkey agar plates and incubated at 37 °C for 48 hours.